دانلود رایگان مقاله لاتین کرنش چرخه ای ترویج نژاد تفکیک استئوژنیک از سایت الزویر
عنوان فارسی مقاله:
کرنش چرخه ای ترویج نژاد تفکیک استئوژنیک از یک سلول استرومایی استخوان مغز و سیستم هم فرهنگی سلول اندوتلیال عروقی
عنوان انگلیسی مقاله:
Cyclic tensile strain promotes the osteogenic differentiation of a bone marrow stromal cell and vascular endothelial cell co-culture system
سال انتشار : 2016
برای دانلود رایگان مقاله کرنش چرخه ای ترویج نژاد تفکیک استئوژنیک اینجا کلیک نمایید.
مقدمه انگلیسی مقاله:
1. Introduction
It is well-established that neovascularization plays a crucial role in the bone formation process during development and fracture healing [1,2]. Newly formed vasculature provides bone tissue with nutrients, oxygen, and growth factors and removes wastes. Substantial evidence has indicated that vascular endothelial cells (VECs) promote the osteogenic differentiation of bone marrow stromal cells (BMSCs) via direct cell-to-cell contact or diffusible molecules [3,4]. Thus, the co-culture of VECs and BMSCs has become a common approach to achieve pre-vascularization for tissue-engineered constructs [2]. In addition to vascularization, mechanical loading is also closely coupled to the rate and quality of bone formation [5e7]. Bone mass and architecture are dynamically adapted to external loading. Previous studies have analyzed the responses of osteoprogenitors, including BMSCs, under cyclic mechanical strain and have demonstrated that such tensile loading enhances BMSC osteogenesis [8e10]. Because both VECs and mechanical stimuli are involved in bone formation and remodeling, the combined treatment of both factors may have a more significant impact on bone regeneration compared to a single factor alone. Several previous studies have focused on this combined effect and suggested that the presence of VECs with mechanical stimuli could further enhance BMSC osteogenesis [7,11]. However, optimized culture conditions and the intrinsic mechanism of this combined effect remain to be further elucidated. Current concepts suggest that paracrine and autocrine mechanisms involving various diffusible molecules are essential in regulating the metabolic activities of co-cultured cells [4]. Among these diffusible molecules, vascular endothelial growth factor (VEGF) is a potent angiogenic and osteogenic promotor that activates its receptor 2 (VEGFR2) both in vitro and in vivo [12]. Moreover, several studies have noted VEGF level elevations induced by various mechanical stimuli in mono-cultured BMSCs, osteoblasts or other osteoprogenitors [10,13,14]. Based on the aforementioned, we hypothesize that VEGF is a key regulator in the cross-talk between BMSCs and VECs under cyclic mechanical strain. The goal of this study was to investigate the combined effect of VECs and cyclic mechanical strain on BMSC osteogenesis in vitro while elucidating the regulatory mechanism of this co-culture system. To accomplish this, a 2-dimensional (2D) direct contact co-culture system of rat BMSCs and VECs was established and then subjected to equibiaxial cyclic tensile strain. The optimal co-culture cell ratio and mechanical loading magnitude were determined based on the best osteogenic effect. The specificity of BMSC differentiation was assessed by examining markers of osteogenic differentiation. Furthermore, the role of VEGF as a candidate regulator was evaluated via specific receptor inhibitors. The results confirmed that cyclic tensile strain further enhanced BMSC osteogenesis in the BMSC/VEC co-culture system and indicated that VEGF plays an essential role in the cross-talk between VECs and BMSCs under dynamic loading conditions via paracrine regulation.
برای دانلود رایگان مقاله کرنش چرخه ای ترویج نژاد تفکیک استئوژنیک اینجا کلیک نمایید.
کلمات کلیدی:
Cyclic tensile strain promotes the osteogenic differentiation of a bone ... https://www.researchgate.net/.../307109508_Cyclic_tensile_strain_promotes_the_osteog... Cyclic tensile strain promotes the osteogenic differentiation of a bone marrow stromal cell and vascular endothelial cell co-culture system. Article in Archives of ... Differentiation and expansion of endothelial cells from human bone ... onlinelibrary.wiley.com/doi/10.1046/j.1365-2141.2001.03077.x/pdf by N Quirici - 2001 - Cited by 484 - Related articles vascular grafts, the contribution of mature endothelial cells to this process is ... existence of a bone marrow-derived endothelial cell precursor was ... CD341 cells co-expressing VEGFR-2 and the novel .... Cultures of CD1331 bone marrow cells. CD1331- .... the ECs and stromal layers or in a transwell insert with a. 0´4-mm ... PHBV/bioglass composite scaffolds with co-cultures of endothelial ... pubs.rsc.org/en/content/articlehtml/2017/RA/C7RA02767B Apr 21, 2017 - ... scaffolds with co-cultures of endothelial cells and bone marrow stromal cells ... between bone vascular endothelium and osteoblastic cells (OCs) and .... of PHBV/BG scaffolds on osteogenic differentiation of co-cultured cells, ... Bioreactor Systems for Tissue Engineering II: Strategies for the ... https://books.google.com/books?isbn=3642160514 Cornelia Kasper, Martijn van Griensven, Ralf Pörtner - 2010 - Science Strategies for the Expansion and Directed Differentiation of Stem Cells ... gene regulation in an endothelial cell-osteoblast co-culture model. ... of vascular endothelial growth factor in bone marrow stromal cell modulation of endothelial cells. The Biology and Therapeutic Application of Mesenchymal Cells - Set https://books.google.com/books?isbn=1118907515 Kerry Atkinson - 2017 - Medical Mesenchymal stromal cells transmigrate over the endothelial barrier. ... migration of human mesenchymal stromal cells across bone marrow endothelial cells. ... endothelial progenitor cell/mesenchymal stem cell interaction in co-culture and its ... Vascular endothelial growth factor stimulates endothelial differentiation from ...
