دانلود رایگان مقاله لاتین سرطان روده بزرگ از سایت الزویر
عنوان فارسی مقاله:
تشخیص غیر تهاجمی و بسیار حساس جهش سرطان روده بزرگ با استفاده از تعیین توالی تک مولکول نسل سوم
عنوان انگلیسی مقاله:
Highly sensitive, non-invasive detection of colorectal cancer mutations using single molecule, third generation sequencing
سال انتشار : 2015
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مقدمه انگلیسی مقاله:
1. Introduction
Although the rate of colorectal cancer (CRC) has been declining at 3.0% per year over the past decade, in 2014 there were about 140,000 new cases in the United States making it the third most common cancer after lung-bronchus and prostate (Cancer Facts and Figures, American Cancer Society, 2014) and this figure in North America essentially mirrors the latest available worldwide survey (www.globocan.iarc.fr). A substantial amount of research has been conducted in the past thirty years to demonstrate that the molecular genetic landscape of CRC is extremely complex. Single Nucleotide Polymorphisms (SNPs), Insertion–Deletions (Indels), Microsatellite Instabilities (MSI), and alteration in methylation patterns can all occur at different loci depending on the site of tumor and its stage (Fearon, 2011; The Cancer Genome Atlas Network, 2012; Tomlinson et al., 2010; Houlston, 2012). The molecular mechanisms behind the formation of adenomas and their progression into CRC were first presented 25 years ago in a model proposed by Fearon and Vogelstein (Fearon and Vogelstein, 1990). Briefly, early adenomas emerge on the normal epithelium and this event is associated with mutations in the Adenomatous Polyposis Coli (APC) or β-catenin (CTNNB1) genes (Morin et al., 1997; Sparks et al., 1998). Most early adenomas suffer additional mutations in either the Kirsten Rat Sarcoma (KRAS) or v-raf murine sarcoma viral oncogene homolog B (BRAF) genes (or other genes of the RTK-RAS pathway), which lead to the formation of intermediate and larger adenomas (Chan, 2003). It is at this point that chromosomal instabilities (CIN) or deficiency in the mismatch repair (MMR) system begin to occur, which leads to an increased mutation rate in the neoplastic cells. CIN in colorectal cancer is often observed on the long arm of chromosome 18 and it is associated with mutations in genes SMAD2 and SMAD4 (Takagi et al., 1996; Miyaki et al., 1999). These genes represent human homologs of mothers against decapentaplegic (MAD) in drosophila and of the SMA protein in Caenorhabditis elegans. On the other hand, MMR is usually caused by a decrease in the expression of the MutL-homolog 1 (MLH1) gene on chromosome 3 through the hyper methylation of the promoter region (Vilar and Gruber, 2010). MMR induces alteration of microsatellite sequences in the tumor cells and increases the overall mutation rate for all genes, including oncogenes and tumor suppressor genes (Parsons et al., 1993). Finally, the progression to carcinoma is often accompanied by the malfunctioning of the cellular tumor antigen TP53, a protein with tumor suppressor activity.
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کلمات کلیدی:
Highly sensitive, non-invasive detection of colorectal cancer mutations ... www.pacb.com/.../highly-sensitive-non-invasive-detection-of-colorectal-cancer-mutat... PacBio's Single Molecule, Real-Time (SMRT) Sequencing offers the most ... of colorectal cancer mutations using single molecule, third generation sequencing. Single molecule targeted sequencing for cancer gene mutation detection https://www.nature.com › Scientific Reports › Articles by Y Gao - 2016 - Cited by 2 - Related articles May 19, 2016 - Single molecule targeted sequencing for cancer gene mutation detection ... in a single disease-targeted clinical test using next generation sequencing. ..... 13) and BRAF (V600E) in colorectal cancer predict poor prognosis and ... Third, a ddNTP labelled with Cy3 is ligated to the denatured DNA fragment. High sensitive detection of colorectal cancer mutations using third ... www.ashg.org/2013meeting/abstracts/fulltext/f130120731.htm Colorectal cancer (CRC) is a major cause of cancer mortality. ... Conclusions: We performed third generation, single molecule sequencing to detect low ... [PDF]Highly sensitive, non-invasive detection of colorectal cancer mutations ... iranarze.ir/wp-content/uploads/2016/10/E212.pdf using single molecule, third generation sequencing ... Colorectal cancer (CRC) represents one of the most prevalent and lethal malignant neoplasms and every ... [PDF]High sensitivity, non-invasive detection of colorectal cancer mutations ... https://www.ethz.ch/content/...genomics.../GENOMICS_2014_ASHG_Russo.pdf by G Russo - Related articles CRC. Methods : • PacBio RS is a single molecule, 3rd generation sequencing technology; it generates highly accurate reads on short amplified fragments using ... Highly sensitive, non-invasive detection of colorectal cancer mutations ... www.fgcz.ch/research/CRC_detection.html Highly sensitive, non-invasive detection of colorectal cancer mutations using single molecule, third generation sequencing. Main content. Colorectal cancer ... [PDF]Mutations by Next Generation Sequencing in Stool DNA from ... www.lifescienceglobal.com/pms/index.php/jao/article/download/3736/2189 by O Youssef - 2016 - Related articles Apr 5, 2016 - Generation Sequencing of stool specimens from patients with gastrointestinal tumors. ... Keywords: Mutation, DNA, Stool specimen, Colorectal carcinoma, Next generation sequencing. 1. .... and high sensitivity of single molecule third generation ... cancer genes from fecal DNA using the Ion Torrent. Patent WO2014093186A1 - Non-invasive detection of colorectal ... www.google.com/patents/WO2014093186A1?cl=en Jun 19, 2014 - When a patient has a colorectal tumor, mutated DNA from the tumor can be ... The difficulty with these approaches of screening for tumors using DNA from ... Single Molecule Real Time (SMRT) sequencing performed on circular ... Second generation sequencing (Ion Torrent, lllumina, and 454) can obtain ...
