دانلود رایگان مقاله لاتین خواص تولید agarase از سایت الزویر
عنوان فارسی مقاله:
خواص تولید agarase خارج سلولی توسط اسینتوباکتر junii PS12B جدا شده از رسوبات دریایی
عنوان انگلیسی مقاله:
Characterization of extracellular agarase production by Acinetobacter junii PS12B, isolated from marine sediments
سال انتشار : 2016
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مقدمه انگلیسی مقاله:
1. Introduction
Most of the marine microbes have evolved degradation enzymes to use agar or agarose as an energy and carbon source (Pluvinage et al., 2013) Marine bacteria secrete a specific glycoside hydrolases (GH) enzyme agarase to utilize algal cell wall polysaccharides as a carbon and energy source. Agarases are classified based on the mode of action, into α-agarase (EC 3.2.1.158), which cleaves α-1, 3 linkages to produce agaro-oligosaccharides and β- agarase (EC 3.2.1.81) which cleaves β-1, 4 linkages to produce neoagaro-oligosaccharides. β-Porphyranases hydrolyze the β-(1, 4) glycosidic bonds of the porphyran moieties (G-L6S) in agar and produces oligosaccharides with reducing ends at G residue (Hehemann et al., 2010). Based on the amino acid sequence similarity, β-agarases are found in four distinct glycoside hydrolases (GH) families in the CAZy database includes GH16, GH50, GH86, and GH118, whereas α-agarase belong to GH96. To date, a vast number of agarolytic micro-organisms from taxonomically diverse genera, as well as agarases and their encoding genes have been well reported and summarized (Chi et al., 2012; Fu et al., 2010; Michel et al., 2006). The approaches to increase the productivity of agarases by agarolytic organisms would be to isolate hyper-producers or mutants for agarases or by cloning the genes encoding for agarases and expression of these genes by molecular genetic techniques. Agarase producing bacteria has been isolated from different environments. Most of the agarase producing isolates were found to be of marine origin. However, Feng et al. (2012) isolated an agarolytic Rhodococcus sp. from printing and dyeing wastewater. Agarolytic bacteria have also been isolated from terrestrial soil (Suzuki et al., 2003; Hosoda et al., 2006; Lakshmikanth et al., 2006b). In recent years, several novel agarase producing strains were isolated from marine environments. Agarolytic Aliagarivorans marinus and Aliagarivorans taiwanensis (Jean et al., 2009), Simiduia agarivorans (Shieh et al., 2008) were isolated from seawater. Flammeovirga sp. MY04 (Han et al., 2012), Agarivorans sp. (Hu et al., 2009) isolated from marine sediments were found to produce β–agarase. Seaweeds were found to be one of the primary sources of agarolytic bacteria in the marine environment (Lee et al., 2013; Oh et al., 2010). Agarolytic bacteria have also been isolated from the gut of mollusks (Fu et al., 2008; Jung et al., 2012). Applications of agarases are well-known in the areas of food, pharmaceuticals, cosmeceuticals, and biotechnology. The neoagarooligosaccharides have been considered to have high economic value, because of their physiological and biological activity without toxicity, as GRAS. These oligosaccharides especially neoagarotetraose and neoagarohexaose exhibit antioxidative activity, scavenging hydroxyl free radicals and superoxide anion radicals and inhibiting lipid peroxidation (Wang et al., 2004; Wu et al., 2005). Additionally, neoagarooligosaccharides acts as a low-calorie
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کلمات کلیدی:
Characterization of extracellular agarase production by Acinetobacter ... https://www.infona.pl/.../bwmeta1.element.elsevier-3d39db9c-a820-3425-90f6-d75a149... by TL Roseline - 2016 - Related articles A marine, gram negative, rod shaped bacterium that degrades agar was isolated from the east coast of India and was identified to be Acinetobacter junii PS12B ... Production and purification of agarase from a ... - Wiley Online Library onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03990.x/full by Z Hu - 2009 - Cited by 51 - Related articles Dec 1, 2008 - Methods and Results: An agarase-producing bacterium strain ... Strain HZ105 produced three extracellular agarases which were ..... Acinetobacter sp. ..... of extracellular agarase production by Acinetobacter junii PS12B, ... Production and purification of agarase from a ... - Wiley Online Library onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2008.03990.x/abstract by Z Hu - 2009 - Cited by 51 - Related articles Dec 1, 2008 - Effect of pH, NaCl on the growth and agarase production of strain HZ105 was studied. Strain HZ105 produced three extracellular agarases ... Purification and Characterization of Catalase from Marine Bacterium ... https://www.hindawi.com/journals/bmri/2014/409626/citations/ May 27, 2014 - ... Characterization of Catalase from Marine Bacterium Acinetobacter sp. ... of extracellular agarase production by Acinetobacter junii PS12B, ... Biocatalysis and Agricultural Biotechnology | Vol 6, Pgs 1-226, (April ... sciencedirect.wswhe.orc.scoolaid.net/science/journal/18788181/6 Process optimization for hyper production of xylanase via statistical .... of extracellular agarase production by Acinetobacter junii PS12B, isolated from marine ... Biocatalysis And Agricultural Biotechnology RSS Page 4 - MedWorm https://medworm.com/rss/search.php?qu=Biocatalysis+and...t...s... The glucanase enzyme showed effective hydrolysis of glucan produced by .... of extracellular agarase production by Acinetobacter junii PS12B, isolated from ...