دانلود رایگان مقاله لاتین تخم آئدس اجیپتی از سایت الزویر


عنوان فارسی مقاله:

تمایز تخم آئدس اجیپتی و آادس notoscriptus (دوبالان: کولیسیده) با استفاده از میکروسکوپ الکترونی روبشی


عنوان انگلیسی مقاله:

Differentiation of Aedes aegypti and Aedes notoscriptus (Diptera: Culicidae) eggs using scanning electron microscopy


سال انتشار : 2016



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مقدمه انگلیسی مقاله:

1. Introduction

Aedes notoscriptus mosquitoes are capable of transmitting Ross River virus (RRV), Barmah Forest virus (BFV), and Rift Valley fever (RVF) (Sota and Mogi, 1992; Turell and Kay, 1998; Watson and Kay, 1998, 1999; Watson et al., 2000; Harley et al., 2001). Ae. aegypti is a known vector of dengue and is also a potential vector of RRV (Watson and Kay, 1999; Harley et al., 2000). Ae. aegypti distribution is limited to tropical and sub-tropical environments while Ae. notoscriptus is found throughout the south-west Pacific islands and in all states of Australia, including many Australian off-shore islands (Lee et al., 1982). While the two species are bionomically similar, the only region of Ae. notoscriptus and Ae. aegypti sympatry in Australia is in tropical north-east Queensland, where the two species appear to coexist in equilibrium (Tun-Lin et al., 1999). Ae. notoscriptus and Ae. aegypti are both peri-domestic, container-breeding mosquitoes, laying their eggs at or above the water line on the edge of natural and artificial receptacles, where eggs may remain dry between inundations. This ability to resist desiccation enables persistence of the species within their current ranges (Kearney et al., 2009; Williams et al., 2010). Mosquito egg desiccation resistance has also been strongly correlated with egg size, suggesting morphological bases for the trait (Sota and Mogi, 1992). Comparative intraspecific egg studies of several anopheline mosquitoes have revealed variations in egg surface morphology which have been correlated with geographical differentiation of the populations (Linley et al., 1993a, 1996; Lounibos et al., 1999). Furthermore, scanning electron microscopy (SEM) of eggs of four strains of the Culex quinquefasciatus species complex revealed variation in egg morphometrics, suggesting an influence of ecological variation (Suman et al., 2009). The chorion structure has been useful in distinguishing some Stegomyia mosquito species, through comparative SEM analyses (Matsuo et al., 1974; Linley, 1989). Phylogenetic analyses of geographically distinct Ae. notoscriptus populations have recently suggested the species is rather a complex of genetic lineages (Endersby et al., 2013). Similarly, allelic variation has been noted in Australian populations of Ae. aegypti (Endersby et al., 2009). SEM analysis of Ae. notoscriptus eggs obtained from Sydney, Australia (Linley et al., 1991) and Ae. aegypti from Florida (Linley, 1989) have previously been conducted, however, inter- and intraspecific comparisons or statistical differentiations have not been made. In the present study, we utilised SEM to analyse the eggs of two Australian populations of both Ae. aegypti and Ae. notoscriptus from geographically and climatically distinct regions, to enable inter- and intraspecific comparisons of the egg morphology and ultra-structural morphology. We hypothesised that there would be significant differences in the eggshell morphology of these two mosquitoes, given they are distinct species, with variable geographic distribution. Eggshell analyses conducted using SEM will contribute to our understanding of invasive container-breeding mosquito ecology and eggshell morphology



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کلمات کلیدی:

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