دانلود رایگان مقاله لاتین اکسید نیتریک میتوکندری از سایت الزویر
عنوان فارسی مقاله:
تولید اکسید نیتریک میتوکندری حمایت شده توسط انتقال الکترون معکوس
عنوان انگلیسی مقاله:
Mitochondrial nitric oxide production supported by reverse electron transfer
سال انتشار : 2016
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مقدمه انگلیسی مقاله:
1. Introduction
In mitochondria, nitric oxide (NO) is synthetized from L-arginine, NADPH and O2 in a reaction catalyzed by mitochondrial nitric oxide synthase (mtNOS) [1,2]. Giulivi and coworkers [3] sequenced rat liver mtNOS and identified the enzyme as an inner membrane integral protein and as the a-splice variant of the nNOS transcript, myristoylated and phosphorylated. Decisive evidence was provided by Kanai et al. [4] with the electrochemical determination of Ca2þ-induced NO release from a single mouse heart mitochondrion, a process that was abolished in nNOS/ knockout mice. NO production by heart mitochondria has been observed in mitochondrial membranes [5,6] and in coupled mitochondria [7,8]. Finocchietto et al. [9] have shown that the NO produced by translocated nNOS (mtNOS) is the insulin-dependent signaling molecule that controls mitochondrial O2 utilization. Mitochondrial NO production accounts for about 60% of total heart NO generation [6] and it is produced by mitochondrial membranesat a rate of 1.0e1.5 nmol NO. min1 mg protein1 and kept at a steady state level of 200e350 nM in the mitochondrial matrix [7,8,10]. At sub-micromolar concentrations, NO is a physiological regulator of mitochondrial functions [1,2,8] through two main effects on the mitochondrial respiratory chain: the competitive inhibition of cytochrome oxidase (complex IV) [11e13] and the inhibition of electron transfer between cytochromes b and c at complex III [14e16]. In addition, NO participates in the intramitochondrial diffusion limited reaction in which NO itself and superoxide anion (O2 - ) react to produce peroxynitrite (ONOO) [17]. Nitric oxide production by rat liver and brain mtNOS has been associated with complex I activity [18]. This finding agrees with the results obtained by Franco et al. [19] who have shown that complex I proteins from rat liver mitochondria co-immunoprecipitate with mtNOS. The association between mtNOS and complex I and IV proteins is compatible not only with the concept of respiratory chain supercomplexes formation with strong protein-protein interactions [20e23], but also with the dependence of mtNOS activity on the metabolic states and membrane potential [7,8,10]. Mitochondrial complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of two electrons from NADH, via flavin mononucleotide (FMN) and a series of iron-sulfur centers (Fe-S) to ubiquinone (UQ) in a reaction associated with proton translocation across the inner membrane, contributing to the protonmotive force (Dp) [24,25]. Mitochondrial complex I reaction is reversible: the complex is able to reduce NADþ in the presence of succinate, in a thermodinamically non spontaneous reaction, associated to the energy of ATP hydrolysis. The physiological occurrence of the reaction was early recognized by Chance and Hollunger [26] and by Klingenberg and colleagues [27] and was termed reverse electron transfer (RET). Complex I is then a reversible enzyme [28,29] that switches sharply between the forward and the reverse reactions through a point at which the free energy of the redox reaction is equal and opposite to that for proton tranlocation [30]. The point of equilibrium makes the reaction as thermodynamically reversible and energetically efficient. Co
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کلمات کلیدی:
Mitochondrial ROS Produced via Reverse Electron Transport Extend ... www.cell.com/cell-metabolism/abstract/S1550-4131(16)30112-7 by F Scialò - 2016 - Cited by 20 - Related articles Apr 12, 2016 - Mitochondrial ROS Produced via Reverse Electron Transport Extend Animal Lifespan ... Open access funded by European Research Council ... Generation of reactive oxygen species by the mitochondrial electron ... onlinelibrary.wiley.com/doi/10.1046/j.0022-3042.2002.00744.x/pdf by Y Liu - 2002 - Cited by 941 - Related articles complex I through reversed electron transfer, not at the ubiquinone of complex .... supported ROS production occurs at the ubiquinone site of complex III (Turrens ... Evidence for Two Sites of Superoxide Production by Mitochondrial ... www.jbc.org/content/286/31/27103.full by JR Treberg - 2011 - Cited by 109 - Related articles Aug 5, 2011 - Superoxide production during reverse electron transport has been ... Support for the two-site model comes from three key observations: (i) there ... Reactive oxygen species production by forward and reverse electron ... https://www.ncbi.nlm.nih.gov/pubmed/21483483 by VA Selivanov - 2011 - Cited by 74 - Related articles Mar 31, 2011 - Reactive oxygen species (ROS) produced in the mitochondrial respiratory ... and the dynamics of NAD(+) reduction by reverse electron transport from ... Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't ... [PDF]Author's personal copy - ResearchGate https://www.researchgate.net/...production.../55dacc0208aeb38e8a8a2104.pdf Fatty acids as modulators of the cellular production of reactive oxygen species. Peter Schönfeld a,⁎ ..... ROS production supported by reverse electron transport. Metal Ions in Neurological Systems - Page 145 - Google Books Result https://books.google.com/books?isbn=3709110017 Wolfgang Linert, Henryk Kozlowski - 2012 - Medical 9 Forward (F.E.T.) and reverse (R.E.T.) electron transfer in the respiratory chain ... reactions supporting ROS production was the reverse electron transfer (RET), ... Electron transport chain - Wikipedia https://en.wikipedia.org/wiki/Electron_transport_chain An electron transport chain (ETC) is a series of complexes that transfer electrons from electron ... The function of the electron transport chain is to produce a transmembrane proton electrochemical gradient as a result of the .... This process of reverse electron transport is important in many prokaryotic electron transport chains. Searches related to production supported by reverse electron transfer reverse electron transport chain reverse electron transfer mitochondria reverse electron flow chemolithotrophs reverse electron transport purple bacteria rotenone
