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عنوان فارسی مقاله:
ماتریس MWCNT بر اساس عنوان پلت فرم برای چسبندگی و رشد سلول
عنوان انگلیسی مقاله:
MWCNT based matrices as a platform for adhesion and growth of cells
سال انتشار : 2016
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مقدمه انگلیسی مقاله:
1. Introduction
New nanomaterials for tissue engineering applications are highly demanded and still drive much attention. One of major approaches in this area, and in regenerative medicine, is the application of carbon-based nanomaterials, including graphene and carbon nanotubes, in different shapes and forms. Several types of carbon-based matrices have been proposed so far i.e. MWCNTs platform based on polymer, ones with specified cavities size, carbon based nanocomposites [1]. MWCNTs dispersed in polymer matrixes, aligned MWCNTs [2] and many others. The main reason to implement carbon nanostructures into scaffolds is to improve the mechanical properties by increasing the Young modulus and tensile strength of the structure. Furthermore, the combination of carbon nanomaterials with substrates support cell attachment, growth and differentiation [3]. Moreover, carbon nanomaterials can be biologically functionalized with various molecules to design scaffolds for tissue growth [4,5]. CNTs show a high binding affinity to biomolecules, including extracellular matrix (ECM) proteins, e.g fibronectin. Interestingly, authors proposed fibronectin e carbon nanotubes composite (hybrid nanostructure) as a pattern (matrix) for controlled cell adhesion and growth of various cells [6,7]. Recently, the interactions of CNTs with cells have been studied, for example adhesion, morphogenesis, proliferation and differentiation [8e11]. The adhesion and proliferation of different type of cells (include gingival fibroblasts) have previously been studied on various surfaces (e.g., on polystyrene, glass and titanium, hydroxyapatite/collagen composites) [12,13]. Many reports highlight the interaction between CNTs and different types of cells [14]. During in vitro studies, a good adhesion and proliferation of the investigated cells has been observed [15]. It has been found that CNTs combined with several materials (e.g., biopolymers) improve cell contact and adhesion [16]. CNT based nanomaterials allow for the quick adaptation of cells to a new environment by modulating the expression of a key protein involved in adhesion (integrin), as well as providing stronger adhesion and survival [17]. Aoki et al.reported osteoblast-like cell proliferation, showing that alkaline phosphatase activity and protein adsorption on CNT based substrates are higher than on polycarbonate membranes and graphite, as a result cell growth on CNTs greatly extended in all directions [18]. Previous studies report that CNT based matrices can be also applied to enhance the growth and differentiation of neurons [19]. One of approaches is to use MWCNT based structures as scaffolds for tissue engineering, for example potential biomaterial in bone reconstruction. It has been shown that carbon nanotube composite scaffolds are biocompatible materials with sufficient mechanical strength for the repair and regeneration of bone [20]. Different groups of nanomaterials can be considered in term of using them in biomedical applications i.e. individual CNTs, CNTs filled with magnetic nanoparticles, CNT based carpets. Due to their unique physical, chemical and mechanical properties as well as the possibility of their biofunctionalization, CNTs can be potentially applied in drug delivery systems, bio-imaging, contrast agents, gene carriers and as implants [21e23]. However, the surface modification and cytotoxicity of carbon nanomaterials are a crucial points in case of cell adhesion, therefore biocompatibility control. In the scientific literature there is still many contradictory results about cytotoxicity of CNTs [24]. In recent years, CNT carpets have gained attention in in vitro studies due to their high processing capability, mechanical stability, low cytotoxicity and parallel alignment [25]. Due to their potential application for tissue engineering, it is important to study the cell adhesion and growth on different sorts of MWCNT matrices, i.e. pristine MWCNT based carpers or covalently modified. Well-aligned MWCNT based matrices, i.e. MWCNT carpets, enable them to be used in regenerative medicine and tissue engineering [26]. The distribution of functionalized MWCNTs in the scaffold structures enhances surface hydrophilicity and provides a preferable microenvironment for cell attachment [27]. Mainly, MWCNT based carpets are synthesized by the Floating Catalyst Chemical Vapor Deposition (FCCVD) route on a quartz substrate typically using a metallocene-hydrocarbon solution [28]. This method allows control over the size, shape and alignment of MWCNT based matrices. In recent years, scientists have drawn attention to the application of carbon materials in dentistry and oral regenerative medicine, thus composites are more tough and can be easily employed as implants. Since MWCNTs might be produced in different forms, they can be used as a new class of implants. Previous research has concluded that CNT functionalized with sodium hyaluronate (HY-SWCNT) may be a potential biomaterial for application in dentistry in regenerative therapies, i.e. for tooth socket repair [29]. Dental filling (glass ionomer cement) with incorporated MWCNTs for dental restorations has revealed high strength and hardness and reduced loss due to friction and wear [30]. Due to the increasing attention of MWCNT based matrices in dentistry and tissue engineering, it is necessary to study various cellular mechanisms, for example adhesion and proliferation. In this work, we synthesized, modified and characterized the surface of MWCNT based carpets in terms of their potential usefulness in cell culturing and tissue engineering. Annealing at 1900 C, purification in hydrochloric acid (HCl) and oxidation in Sulfuric and Nitric acid were conducted in order to change the MWCNT morphology and surface. We investigated the effect of MWCNTs on the viability of human gingival fibroblast (HGF-1) and human bone osteosarcoma epithelial (U2OS) cell lines. Furthermore, we studied the interaction of cells with the potential scaffolds by using Scanning Electron Microscopy, Atomic Force Microscopy and Confocal Microscopy. We have found that the Factin cytoskeleton organization modulates the MWCNT matrices surface. We also discussed their capability for future orthopedics and dental implants as well as tissue engineered scaffolds.
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کلمات کلیدی:
11. MWCNT based matrices as a platform for adhesion and ... - 首页 en.ustc.findplus.cn/?h=articles&db=edselc&an=edselc.2...0... Translate this page Title: MWCNT based matrices as a platform for adhesion and growth of cells. Authors: Warowicka, A.1. Maciejewska, B.M.1. Litowczenko, J.1. Kościński, M.1,2 Online solid sampling platform using multi-wall carbon nanotube ... pubs.rsc.org/en/content/articlepdf/2015/ja/c4ja00436a?page=search Jan 9, 2015 - multi-wall carbon nanotubes (MWCNTs) assisted matrix solid phase ... Hg2+ and CH3Hg+, respectively, based on 1 mg of fish sample. Online solid sampling platform using multi-wall carbon nanotube ... pubs.rsc.org/-/content/articlehtml/2015/ja/c4ja00436a by D Deng - 2015 - Cited by 12 - Related articles Jan 9, 2015 - Online sequential injection solid-phase matrix dispersion platform .... Therefore, MWCNTs-based MSPD was coupled to HPLC-ICP-MS for the ... Advances in Heterogeneous Material Mechanics 2011: Proceedings of ... https://books.google.com/books?isbn=1605950548 Jinghong Fan, Junqian Zhang, Haibo Chen - 2011 - Technology & Engineering wall in a MWCNT is affected at most by its two adjacent walls. ... the MM simulations on the pull-out behavior of CNT from PE matrix are carried out. ... Based on our previous definition for the platform stage II [12], i.e., subtracting 2a=1.0 nm ... Development of an efficient immobilization matrix based on a ... pubs.rsc.org/-/content/articlehtml/2014/tb/c3tb21356k by S Soylemez - 2014 - Cited by 16 - Related articles Nov 19, 2013 - Development of an efficient immobilization matrix based on a ... Carboxylic acid functionalization of MWCNTs was carried out via acid treatment. ... as well as well-organized suitable platform for enzyme immobilization.
