دانلود رایگان مقاله لاتین میکروکپسول از سایت الزویر
عنوان فارسی مقاله:
میکروکپسول - درهم بافته ساختاربندی شده: داربست جدید برای عدم تحرک آنزیم
عنوان انگلیسی مقاله:
Structured interlocked-microcapsules: A novel scaffold for enzyme immobilization
سال انتشار : 2017
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مقدمه انگلیسی مقاله:
1. Introduction
In recent years, mesoporous microcapsules have been widely concerned owing to their combination of hollow structures and mesoporous nanostructures [1–3]. Some of them like mesoporous silica nanoparticles included SBA, MCM and MSU have been widely used in preparing catalysts [4]. Due to their superior physicochemical properties, mesoporous microcapsules are particularly pursued as enzyme immobilization supports [5,6]. In general, both the microcapsule wall and the capsule lumen can be used for immobilizing enzyme. But up to now, most of the research encapsulated enzyme molecules in the capsule lumen, and the capsule wall only plays the role of preventing the leakage of enzyme molecules. What's more, the dense, thick capsule wall and the stagnant solution in the capsule lumen would cause the high transfer resistance between the capsule lumen and the bulk solution, and then the reaction rate would be influenced [7,8]. If the enzyme molecules were immobilized on the capsule wall, once the substrates come in contact with or diffuse through the capsule wall, the catalytic reaction would occur. Thus the catalytic efficiency will be significantly improved by increasing the contact probability of substrate and enzyme and shortening the mass transfer path. In addition, the special physical effects provided by the pores of capsule wall can endow the immobilized enzyme with improved chemical, biochemical, and mechanical properties. Therefore, it is urgent to design novel wall material according to the advanced concept of nanoarchitectonics, which was proposed for the assembly of structural materials at the nanoscale level [9]. Recently, a concept of open-mouthed microcapsules reactor was proposed for electrochemical and gaseous catalytic applications. Like the function of the cell membrane, the structure makes full use of the inner and outer wall of the microcapsule by forming open-mouth on the capsule wall. Benefiting from the unique structure, the capsule lumen is exposed and mass transfer resistance is also decreased [10]. This concept provides a new opportunity for the construction of robust enzyme catalysts. Parallel to the development of the new catalyst pellets with macropores, structuring of the catalyst is also an efficient way to enhance the effective mass diffusivity [11]. The structured catalysts are considered to be a promising research direction in the field of heterogeneous catalysis [12,13]. In our previous study, three-dimensional ordered macroporous biocatalysts were successfully prepared [14], and superior catalytic performance was obtained. Hence, it can be conjectured that setting up a structured biocatalyst composed of open-mouthed microcapsules (or the structured interlocked-microcapsules, SIMC) could enhance the catalytic performance of the immobilized enzyme significantly. However, to the best of our knowledge, no investigation of preparing SIMC for enzyme immobilization has been previously reported. Thus, for the first time, we present a facile approach to prepare novel structured interlocked-microcapsules with interconnected ordered structure for enzyme immobilization. Subsequently, the SIMC was functionalized with amine groups and activated with glutaraldehyde (GA) which is one of the most widely used reagents in enzyme immobilization [15]. GA could reacted with amine groups(both in the carrier or enzyme molecules) rapidly at around neutral condition [16], on this account, the NHase could be
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کلمات کلیدی:
Hierarchical Porous Interlocked Polymeric Microcapsules: Sulfonic ... https://www.nature.com › Scientific Reports › Articles › Article by X Wang - 2017 Mar 16, 2017 - As catalyst supports, we can introduce open mouthed structure and interlocked architecture in the multifunctional polymeric microcapsules. Structured interlocked-microcapsules: A novel scaffold for enzyme ... isi-dl.com/item/138941 ... worldscientific.com. SEARCH. Structured interlocked-microcapsules: A novel scaffold for enzyme immobilization. sciencedirect.com. Source Link ... Construction of Grape-like Silica-Based Hierarchical Porous ... - NCBI www.ncbi.nlm.nih.gov/pubmed/26509289 Nov 4, 2015 - ... Porous Interlocked Microcapsules by Colloidal Crystals Templates. ... whose structure is the subtle integration of open mouthed structure, ... Construction of Grape-like Silica-Based ... - ACS Publications pubs.acs.org/doi/pdf/10.1021/acs.langmuir.5b03465 by J Gu - 2015 - Cited by 2 - Related articles Oct 28, 2015 - like silica-based hierarchical porous interlocked microcapsules. (HPIMs) by ... capsule wall.11 The open mouthed structure and mesoporous. Construction of Grape-like Silica-Based ... - ACS Publications pubs.acs.org/doi/abs/10.1021/acs.langmuir.5b03465 by J Gu - 2015 - Cited by 3 - Related articles Oct 28, 2015 - ... mesoporous microcapsule wall construct the hierarchical porous structure. Furthermore, the interlocked architecture of the microcapsules can ... Construction of Grape-like Silica-Based ... - ACS Publications pubs.acs.org/doi/full/10.1021/acs.langmuir.5b03465 by J Gu - 2015 - Cited by 3 - Related articles Oct 28, 2015 - Furthermore, the interlocked architecture of the microcapsules can ..... (39) Further evidence of the mesoporous structure of HPIMs-SiO2 can be ...
