دانلود رایگان مقاله لاتین فرمالین ثابت مغز انسان برای تحقیق میکروسکوپی الکترونی از سایت الزویر
عنوان فارسی مقاله:
تهیه برشهای فرمالین ثابت مغز انسان برای تحقیقات میکروسکوپی الکترونی
عنوان انگلیسی مقاله:
Preparation of human formalin-fixed brain slices for electron microscopic investigations
سال انتشار : 2016
برای دانلود رایگان مقاله فرمالین ثابت مغز انسان برای تحقیق میکروسکوپی الکترونی اینجا کلیک نمایید.
مقدمه انگلیسی مقاله:
1. Introduction
For morphological investigations of psychiatric disorders, human post-mortem neuronal tissue is of great interest. While empirical research on the malfunctions of these disorders at the cellular level is limited, there is a need to develop novel tools to study the morphology and ultrastructure of neuronal tissue. Such studies could improve our understanding of the cellular pathology of neuropsychiatric disorders, and ultimately help develop more targeted therapeutic strategies. Recently, disrupted in schizophrenia 1 (DISC1) has become a focus of neuropsychiatric disorder investigations. This gene was first described in a Scottish pedigree suffering several psychiatric disorders such as schizophrenia, schizoaffective disorder, uni- and bipolar affective disorder. In this family, DISC1 is disrupted by a balanced translocation (Millar et al., 2000). DISC1 is located within thecytoplasm as well as in the nucleus and provides a wide range of different cellular functions through interactions with other proteins, such as neuronal migration, cell proliferation during development of the nervous system (Ishizuka et al., 2011; Kamiya et al., 2005), neurite outgrowth (Miyoshi et al., 2003; Ozeki et al., 2003), dendritic spine regulation (Hayashi-Takagi et al., 2010), microtubule organisation and finally interaction with microtubule transport proteins (Kamiya et al., 2005). DISC1 is also involved in vesicle transport processes, and in the case of an impaired DISC1 function, the transport mechanisms can be restored using lithium as a therapeutic strategy (Flores et al., 2011). The different functions of DISC1 are well established by immunohistochemistry, but data on ultra-structural alterations is missing. The alteration of cellular structures is a common theme across many neuropsychiatric disorders. In patients with schizophrenia who predominantly display positive symptoms such as delusions or hallucinations, damagedmyelinated fibres in the greymatter can be found. Conversely, in patients with predominantly negative symptoms such as emotional blunting, a degeneration of myelinated sheaths in the white matter is detectable (Uranova et al., 2011). Additionally, patients with schizophrenia suffer a loss of dendrites
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کلمات کلیدی:
A versatile clearing agent for multi-modal brain imaging : Scientific ... https://www.nature.com › Scientific Reports › Articles by I Costantini - 2015 - Cited by 57 - Related articles May 7, 2015 - We characterized the effect of TDE on fixed brain slices in terms of transparency and ... samples we performed transmission electron microscopy (TEM) imaging. .... 2 mm thick block of formalin-fixed tissue removed from the ... Studying synapses in human brain with array tomography and ... www.nature.com › Home › archive › issue › Protocol Jun 20, 2013 - Parallel processing for transmission electron microscopy (TEM) using a .... cutting fixed brain specimens into ribbons of 70-nm serial sections (Fig. ..... for AT and TEM, along with formalin-fixed paraffin-embedded brain tissue ... Processing tissue and cells for transmission electron microscopy in ... www.nature.com › Journal home › Archive › Protocol Oct 4, 2007 - In transmission electron microscopy (TEM), electrons are ... of analysis of tissue already fixed in formalin or processed into paraffin blocks. ... The electron beam passes through an ultrathin plastic section ...... Figure 5: A brain metastasis from an alveolar type II pneumocyte adenocarcinoma of the lung. Optimization of electron microscopy for human brains with long-term ... actaneurocomms.biomedcentral.com/articles/10.1186/2051-5960-2-42 by XB Liu - 2014 - Cited by 10 - Related articles Apr 11, 2014 - The ability to carry out EM on frozen sections allows for investigation of ... In reality, many human brains have been fixed in formalin for long ... Human Polyomaviruses: Molecular and Clinical Perspectives https://books.google.com/books?isbn=0471461024 Kamel Khalili, Gerald L. Stoner - 2004 - Science brain infection. ... (PAP) method in routine histologic sections of formalin-fixed, paraffin-embedded PML brain. ... very useful to investigate large brain sections. ... virions when an adjacent thin section was examined by electron microscopy. Forensic Neuropathology and Associated Neurology https://books.google.com/books?isbn=354028995X Manfred Oehmichen, Roland N. Auer, Hans Günter König - 2006 - Medical The drawback is the risk of artificial wounding to the brain during removal and a ... Molecular biology Electron microscopy Molecular biology Histology (routine) ... 5.1.2.3 Sectioning of the Formalin-Fixed Brain When obtaining brain sections a ...
