دانلود رایگان مقاله لاتین طراحی کندرویتین نانوکمپلکس پلی الکترولیت از سایت الزویر
عنوان فارسی مقاله:
طراحی کندرویتین نانوکمپلکس پلی الکترولیت مبتنی بر سولفات: تشکیل نانوذرات با کیتوزان و یک مطالعه موردی از کلسی تونین سالمون
عنوان انگلیسی مقاله:
Design of chondroitin sulfate-based polyelectrolyte nanoplexes: Formation of nanocarriers with chitosan and a case study of salmon calcitonin
سال انتشار : 2016
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مقدمه انگلیسی مقاله:
1. Introduction
Traditionally, pharmaceutical excipients are treated as “inert” materials and are not expected to have pharmacological activity (Baldrick, 2010), however this view has changed as newly approved excipients cover a range of functions from stabilizing formulations to active roles of enhanced drug uptake and specific drug delivery (Goole et al., 2012). Pharmaceutical polymers bearing a charge, polyelectrolytes, have been extensively studied as components of micro- and nano-sized carriers for the delivery of a range of therapeutic molecules such as peptides and nucleic acids. Among polyelectrolytes, cationic and anionic polysaccharides have received particular attention and some of them also have interesting pharmacological properties. For instance hyaluronic acid (HA) has been shown to act in synergy with salmon calcitonin (sCT) reducing inflammatory biomarkers in vitro and inflammatory arthritis in vivo (Ryan et al., 2013). Another glycosaminoglycan, chondroitin sulfate (CHON) has been used for the preparation of nanocarriers for drug/gene delivery (Zhao, Liu, Wang, & Zhai, 2015). CHON is an unbranched polysaccharide containing twoalternating monosaccharides: d-glucuronic acid and N-acetyl-dgalactosamine. It is an abundant glycosaminoglycan found in cartilage, bone and connective mammalian tissue. CHON is a symptomatic slow-acting agent for osteoarthritis, commonly sold together with glucosamine. It has been shown to be absorbed after oral administration in humans as a high molecular weight polysaccharide (Volpi, 2002), therefore it has a potential to be used to increase the absorption of encapsulated molecules. Coating of chitosan (CHIT) NPs with CHON increased the uptake of the encapsulated nucleic acid by COS7 cells (transformed African green monkey kidney fibroblasts) via interaction of CHON with CD44 receptors (Hagiwara, Nakata, Koyama, & Sato, 2012). CHON has weak, carboxylate, and strong, sulfate, moieties attached to the main glycan backbone. Due to its acidic nature CHON is able to produce ionic complexes with cationic molecules (Denuziere, Ferrier, & Domard, 1996). Examples of such complexes include CHON complexes with protamine (PROT) (Umerska et al., 2015), lysozyme (van Damme, Moss, Murphy, & Preston 1994), trimethylchitosan (Place, Sekyi, & Kipper, 2014), however most of the polyelectrolyte complexes of CHON tested thus far are those with CHIT (Yeh, Cheng, Hu, Huang, & Young, 2011; Tsai et al., 2011; Santo, Gomes, Mano, & Reis, 2012; Place et al., 2014). CHIT is a linear polysaccharide composed of randomly distributed d-glucosamine (deacetylated unit) and N-acetyl-d-glucosamine (acetylated unit)linked via -(1→4)-glycosidic bonds. The properties of CHIT can be useful in medicine, as it reduces bleeding (Pusateri et al., 2003) and has antibacterial activity (Benhabiles et al., 2012). Due to its mucoadhesive properties it could be a valuable component of drug delivery systems (Sogias, Williams, & Khutoryanskiy, 2008). Further research on NPs containing CHON and CHIT is required, as to the best of our knowledge no systematic investigation on the formation of both, positively and negatively charged CHON/CHIT NPs, has been published to date. The charge is of a key importance in cellular uptake and cytotoxicity of medical NPs (Fröhlich, 2012). For instance it has been shown that positively charged HA/CHIT NPs exerted toxic effects on Caco-2 cells in contrast to negatively charged NPs (Umerska et al., 2012). Initially the studies on CHON nanocarriers focused on the employment of CHON as an agent to yield positively charged CHIT NPs intended for the encapsulation of molecules like FITC-BSA (Yeh et al., 2011), BSA (Santo et al., 2012), doxorubicin (Hu et al., 2014), Nell-1 protein (Hou, Hu, Park, & Lee, 2012). The NPs obtained in those studies were characterized by a positive charge. Recently, CHON/CHIT NPs containing CHON as the main ingredient were produced as aggrecan mimicking NPs (Place et al., 2014). However, none of these studies considered the stoichiometry of CHON/CHIT NPs formation. Hence the aim of this paper was to examine the stoichiometry of molecular interactions between CHON and CHIT within CHON/CHIT NPs.Another objective was to discuss the criteria of carrier selection and to select carriers with optimal properties for the encapsulation of a cationic peptide, sCT. Knowing the principles and approach to loading this peptide, the same criteria could be translated to encapsulating similar cationic therapeutically relevant molecules, such as antimicrobial peptides and growth factors. Similarly to HA, CHON NPs are interesting as carriers for sCT due to complementary pharmacological action of both molecules (Umerska et al., 2015). As in some instances the presence of CHIT may not be necessary, and there is evidence that CHON forms complexes with sCT (Umerska et al., 2015), the purpose of this paper was to design and characterize CHON/sCT NPs. Cationic molecules, e.g. CHIT, could compete with sCT for binding with CHON molecules. Because the colloidal stability of polyelectrolyte complex NPs depends on their charge, the incorporation of large quantity of sCT could lead to destabilization of the system. Eliminating cationic CHIT fromthe formulation could offer the advantage of a very high sCT loading. The last objective of this paper was to examine the influence of the composition of the nanocarrier on the stability in different environments and the peptide release.
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کلمات کلیدی:
Design of chondroitin sulfate-based polyelectrolyte nanoplexes - TARA www.tara.tcd.ie/handle/2262/79132 Design of chondroitin sulfate-based polyelectrolyte nanoplexes: Formation of nanocarriers with chitosan and a case study of salmon calcitonin. [PDF]Design of chondroitin sulfate-based polyelectrolyte nanoplexes ... iranarze.ir/wp-content/uploads/2016/11/E2703.pdf Sep 13, 2016 - Design of chondroitin sulfate-based polyelectrolyte nanoplexes: Formation of nanocarriers with chitosan and a case study of salmon calcitonin. Design of chondroitin sulfate-based polyelectrolyte nanoplexes - ISI-dl isi-dl.com/item/90583 Design of chondroitin sulfate-based polyelectrolyte nanoplexes: Formation of nanocarriers with chitosan and a case study of salmon calcitonin. sciencedirect. Design of chondroitin sulfate-based polyelectrolyte nanoplexes ... kti.aficionado.ie/publications/80488 Abstract The aim of this work was to examine the formation and properties of chondroitin sulfate (CHON)-based nanoparticles (NPs), namely CHON/chitosan ... Design of chondroitin sulfate-based polyelectrolyte nanoplexes https://www.sparrho.com/...chondroitin-sulfate-based-polyelectrolyte-nanoplexes-for... Nov 16, 2016 - Design of chondroitin sulfate-based polyelectrolyte nanoplexes: Formation of nanocarriers with chitosan and a case study of salmon calcitonin. Anita Umerska - Google Scholar Citations scholar.google.com/citations?user=M0CPitUAAAAJ&hl=en Post-doc at Universite d'Angers - tcd.ie Lipid-based liquid crystals as carriers for antimicrobial peptides: phase behavior and ... on the process of formation and properties of peptide-loaded nanoparticles ... Design of chondroitin sulfate-based polyelectrolyte nanoplexes: Formation of ... Self-assembled hyaluronate/protamine polyelectrolyte nanoplexes https://wwww.unboundmedicine.com/.../protamine_polyelectrolyte_nanoplexes:_synthes... This work investigates a new type of polyelectrolyte complex nanocarrier ... Design of chondroitin sulfate-based polyelectrolyte nanoplexes: Formation of ... nutraceutical formulations containing glucosamine and chondroitin ... https://innovareacademics.in/journals/index.php/ijcpr/article/view/17380 The role of glucosamine sulfate and chondroitin sulfate in the treatment of .... sulfate-based polyelectrolyte nanoplexes: formation of nanocarriers with chitosan ...
