دانلود رایگان مقاله لاتین سورفاکتانت زیست سازگار از سایت الزویر
عنوان فارسی مقاله:
یک سورفاکتانت زیست سازگار، متیل استر سولفونات (MES)، به عنوان لیگاند رسوب برای تصفیه پروتئین
عنوان انگلیسی مقاله:
A biocompatible surfactant, methyl ester sulphonate (MES), as a precipitating ligand for protein purification
سال انتشار : 2016
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مقدمه انگلیسی مقاله:
1. Introduction
Consumer demand for specialty biotechnology products has driven the need for a more cost effective biomanufacturing process. In addition, downstream processing (DSP) of biomolecules is a major challenge which contributes up to 70% of the overall production cost[1,2]. Although conventional purificationmethods (e.g. chromatography) are known to have a high degree of purification, they also have many drawbacks, e.g., high costs, long processing times, tedious, complex and non-reproducible operation [3]. Therefore, the current trend in DSP is to look for alternative purifi- cation methods which are cheaper and simpler than conventional methods, but with comparable performance. In this context, new approaches like liquid-liquid extraction-based methods, namely reverse-micellar extraction, and aqueous two-phase have been proposed. However, there are also several disadvantages with these ∗ Corresponding author. E-mail address: d.stuckey@imperial.ac.uk (D.C. Stuckey). techniques, such as the degree of product loss, high cost, and complexity/unpredictability of the system [4–7]. Meanwhile, the potential of surfactant precipitation for protein recovery has been extensively reported recently [4,6,8,9–11,12]. Its simplicity, scalability, relative ease of use, high selectivity, not to mention its combined concentration, and purification advantages, has made it appealing in DSP. Work on surfactant precipitation based on the use of conventional surfactants has been substantial [8,9,11,13]. The most important finding was that the charge of the ionic surfactant’s head group is central to precipitation, and this explains why conventional ionic surfactants such as: anionic (AOT) and their cationic counterparts (TOMAC, DODMAC, DTAB and CTAB) have been successfully applied. However, the detergent production industry is focussing on the production of a biobased surfactant due to raised concerns about environmental sustainability [14]. One example of such a detergent is methyl ester sulphonate (MES), a palm oil-derived surfactant [15,16]. The two main features of MES that appeal to the detergent industry are its higher detergency, and environmentally friendly structure. Thus, the overall consumption cost is reduced signifi-
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