دانلود رایگان مقاله لاتین خصوصیات بیوشیمیایی از سایت الزویر
عنوان فارسی مقاله:
خصوصیات بیوشیمیایی و بیوفیزیکی لیشمانیا دونووانی گاما گلوتامیل سنتتاز سیستئین
عنوان انگلیسی مقاله:
Biochemical and biophysical characterization of Leishmania donovani gamma-glutamylcysteine synthetase
سال انتشار : 2016
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مقدمه انگلیسی مقاله:
1. Introduction
Visceral leishmaniasis (VL) is the most devastating and fatal form of leishmaniasis caused primarily by the parasitic protozoan Leishmania donovani, leading to an estimated 300,000 new cases of disease annually with more than 20,000 deaths [1]. Co-infection with HIV results in a significant increase in VL pervasiveness [2]. The disease is prevalent throughout the tropical world and current drug treatment regimen have limitations due to toxicity, high cost, difficult dosage regime and development of resistance to antimonial drugs. These drawbacks have severely reduced the options for clinical treatment of VL and till date no effective drug or vaccine is available. The crucial step in the development of the new drug in a rationale-driven drug discovery approach is the identi- fication, functional and structural characterization of a protein or a pathway in the pathogen that is either absent or sufficiently different from its host. One such pathway is the trypanothione biosynthesis pathway [3–6]. Trypansomatids including Leishmania utilize a potent machinery employing the bis glutathione-spermidine conjugate trypanothione, as a major thiol along with universally present thiol glutathione. In the absence of the ubiquitously present enzymes catalase and glutathione peroxidase which are responsible for neutralizing reactive oxygen species (ROS) in other organisms, trypanothione plays a crucial role in fighting large amount of reactive oxidative species (ROS) generated by the macrophages during the initial phase of parasite–host interactions in trypanosomatids [7–10]. Independently synthesised glutathione and spermidine conjugate to form trypanothione. Glutathione biosynthesis takes place in two steps : the first step, catalysed by γ-glutamylcysteine synthetase (Gcs EC 6.3.2.2) is the ATP-dependent ligation of L-Cysteine to L-Glutamate to form γ-glutamylcysteine [11]; the second step is the conjugation of L-glycine forming glutathione, catalysed by glutathione synthetase in an ATP dependent manner [12]. Spermidine is obtained from putrescine in the reactions catalyzed by ornithine decarboxylase and spermidine synthase. The next two steps resulting in trypanothione biosynthesis are unique to trypanosomatids wherein glutathione couples with spermidine resulting in the formation of glutathionyl spermidine [4], to which
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کلمات کلیدی:
PubMed Result - NCBI https://www.ncbi.nlm.nih.gov/pubmed?db=pubmed&cmd=link&linkname... ... (SAG) sensitive and resistant Indian clinical isolates of Leishmania donovani through ... gamma-glutamylcysteine synthetase and ornithine decarboxylase in natural ... Leishmania donovani: genetic diversity of isolates from Sudan characterized by ... Leishmania donovani isolates: biochemical and biophysical studies and ... [PDF]Biophysical and Pharmacological Characterization of Energy ... journal.frontiersin.org/article/10.3389/fcell.2017.00024/pdf Mar 24, 2017 - field isolated resistant Leishmania strains, but the exact transport pathways ...... Role of ABC transporter MRPA, gamma-glutamylcysteine ... drug resistance in Trypanosoma cruzi and Leishmania donovani by verapamil. Trans. Pragati Agnihotri - Google Scholar Citations scholar.google.com/citations?user=aABnDRIAAAAJ&hl=en Biochemical and biophysical characterization of Leishmania donovani gamma-glutamylcysteine synthetase ... Identification of novel inhibitors of Leishmania donovani γ-glutamylcysteine synthetase using structure based virtual screening, ... Characterization of a Novel Putative S-Adenosylmethionine ... - PLOS journals.plos.org/plosone/article?id=10.1371/journal.pone.0065912 by SP Singh - 2013 - Related articles Jun 19, 2013 - Biophysical studies show that, L. donovani ADL binds ... purification, structural and functional characterization of Leishmania donovani (L. donovani) ...... diphosphatase kinase b and gamma-glutamylcysteine synthetase were ... Substrate Binding Determinants of Trypanosoma brucei γ ... pubs.acs.org/doi/abs/10.1021/bi0159128 by JJ Abbott - 2002 - Cited by 16 - Related articles Feb 1, 2002 - Biochemical and biophysical characterization of Leishmania donovani gamma-glutamylcysteine synthetase. Pragati Agnihotri , Saurabh P. Recent Advances in Redox Active Plant and Microbial Products: From ... https://books.google.com/books?isbn=9401789533 Claus Jacob, Gilbert Kirsch, Alan J. Slusarenko - 2014 - Medical ... Hamilton CJ (2013) Biophysical features of bacillithiol, the glutathione ... (1994) Thiols of intracellular pathogens—Identification of ovothiol-A in Leishmania donovani ... Fahey RC (2003) Characterization of Mycobacterium tuberculosis mycothiol ... The function of gamma-glutamylcysteine and bis-gamma-glutamylcystine ... Vaccination with Recombinant Leishmania donovani Gamma ... www.journalofparasitology.org › Journal of Parasitology › October 2010 by FL Henriquez - 2010 - Cited by 8 - Related articles (2016) Biochemical and biophysical characterization of Leishmania donovani gamma-glutamylcysteine synthetase. Biochemistry and Biophysics Reports 8, ... Exploiting Knowledge on Leishmania Drug Resistance to Support the ... www.cell.com/trends/parasitology/references/S1471-4922(16)30197-0 Dec 16, 2016 - Characterisation of antimony-resistant Leishmania donovani isolates: biochemical and biophysical studies and .... Role of ABC transporter MRPA, gamma-glutamylcysteine synthetase and ornithine decarboxylase in natural ...
