دانلود رایگان مقاله لاتین جلوگیری LH از آپوپتوز ناشی از سیس پلاتین در اووسیت ها از سایت الزویر
عنوان فارسی مقاله:
LH از آپوپتوز ناشی از سیس پلاتین در اووسیت ها جلوگیری کرده و باروری موش های ماده را حفظ می کند
عنوان انگلیسی مقاله:
LH prevents cisplatin-induced apoptosis in oocytes and preserves female fertility in mouse
سال انتشار : 2016
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بخشی از مقاله انگلیسی:
esults
LH protects POs from degeneration induced by Cs in culture. Ovaries from GFP-cKit transgenic mice of 4 days post partum (P4) were cut into small fragments and cultured for 4 days in order to allow spreading of ovarian somatic cells and facilitate the score of the fluorescent oocytes under the microscope; cultures were then exposed for 24 h to two different doses of Cs. We found that the number of the primordial follicle-enclosed oocytes (diameter o20 μm, hereafter termed as primordial oocytes, POs) was severely reduced in the ovarian fragments, incubated in the presence of Cs in comparison with untreated cells (Figures 1a and b) (% morphologically healthy POs, mean±S.E.: Ctrl = 96 ± 1.2; 7.5 μM Cs=54±3.8; 10 μM Cs=33±1.1). Differently, we observed that the few primary/secondary follicleenclosed oocytes (hereafter termed as growing oocytes, GOs) were unaffected by the Cs treatment (Figure 1a). In order to determine whether these results were due to a stagedependent oocyte sensitivity to Cs, we repeated the same experiments on POs and GOs, isolated from P7 to P8 ovaries and freed from follicle cells. The results showed that while only a few denuded POs survived after 24 h in the presence of 10 μM Cs (35/155=22.6%), the large majority of GOs (56- /60=93%) remained healthy as estimated both morphologically and by the Trypan blue test (Supplementary Figure 1A and data not shown). Thus, Cs was able to directly induce death in primordial but not GOs in vitro. Strikingly, we found that pretreatment with LH, in the range of 50–200 mIU/ml (about 0.6–2.3 ng/ml) 1 h before Cs addition, favored in a dose-dependent manner the survival of POs within the ovarian fragments after 24 h of culture, approaching their number to that of the drug-untreated samples (% healthy POs: 7.5 μM Cs±200 mIU LH =80± 3.6; 10 μM Cs+200 mIU LH = 68±3.0) (Figures 1a and b and Supplementary Figure 1B). Noteworthy, LH did not prevent degeneration of POs cultured either freed from pregranulosa cells (data not shown) or enclosed in the follicles isolated from ovarian fragments (Supplementary Figure 1C). Interestingly, we found that LH was also able to sustain the survival of the ovarian somatic cells that was clearly impaired by Cs treatment, as evaluated by morphological observation and the Trypan blue assay (Supplementary Figure 1D). Overall, these results suggest that LH ovoprotection was actually indirect and mediated by a subset of ovarian somatic cells. Finally, we found that also FSH had a slight albeit statistically significant protective effect on the Cs ovotoxicity within the ovarian fragments (Figure 1c), but not on POs within isolated follicles, as described with LH (Supplementary Figure 1C). As LH appeared to exert a significantly much higher ovoprotection than FSH, we focused the subsequent experiments on the LH action. A subset of the somatic cells of early postnatal ovaries express LHR. Western blotting analyses proved that the LHR was expressed at appraisable level both in postnatal ovaries as early as P2 and in the P4 ovarian fragments cultured for 4 days (Figure 2a). RT-PCR analysis confirmed full-length LHR transcripts in the ovarian somatic cells but not in denuded oocytes obtained from P4 ovaries (Figure 2b). In adult and P8 ovaries, LHR was immunolocalized in cells surrounding the growing follicles (presumably theca cells) (Figure 2c). In P4 ovarian fragments cultured for 4 days, a significant number of LHR-positive cells appeared to surround the region containing the primordial and growing follicles (Figure 2c).
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کلمات کلیدی:
Luteinizing hormone inhibits Fas-induced apoptosis in ovarian surface ... joe.endocrinology-journals.org/content/188/2/227.full.pdf by KA Slot - 2006 - Cited by 27 - Related articles Fas-induced apoptosis is also believed to be one of the mechanisms involved in cisplatin cytotoxity in OSE cancer cells (Uslu et al. 1996, Wakahara et al. 1997), a therapy often used as treatment for OSE cancer. We have examined the effect of LH on the occurrence of. Fas-induced apoptosis in the human ovarian epithelial. Golgi-SNARE GS28 potentiates cisplatin-induced apoptosis by ... www.biochemj.org/content/444/2/303 by NK Sun - 2012 - Cited by 14 - Related articles Jun 1, 2012 - Golgi-SNARE GS28 potentiates cisplatin-induced apoptosis by forming GS28–MDM2–p53 complexes and by preventing the ubiquitination and ...... p53 is additionally phosphorylated at Ser46 by DYRK2, for example, which strongly transactivates Bax and induces apoptotic cell death (left-hand side). PAIN – Novel targets and new technologies: https://books.google.com/books?isbn=2889193942 Susan Hua, Peter John Cabot - 2015 - Science (General) Alterations in cell cycle regulation underlie cisplatin induced apoptosis of dorsal root ganglion neurons in vivo. Neurobiol. Dis. ... Acetyl-L-carnitine prevents and reduces paclitaxel-induced painful peripheral neuropathy. Neurosci. ... Oncol. 31, 2627. doi: 10.1200/JCO.2012.44.8738 Hershman, D. L., Weimer, L. H., Wang, ...
