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عنوان فارسی مقاله:
افزایش بیوسنتز بتا کاروتن و تنظیم رونویسی ژن در قارچ بلیکسلیا تریسپورا با استات سدیم
عنوان انگلیسی مقاله:
Enhancing beta-carotene biosynthesis and gene transcriptional regulation in Blakeslea trispora with sodium acetate
سال انتشار : 2016
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مقدمه انگلیسی مقاله:
1. Introduction
The -carotene is a carotenoid of orange-red terpenoid pigments biosynthesized by microorganisms and plants and has been wildly applied in the pharmaceutical, nutraceutical, cosmetic, and animal feed industries [1–4]. The commercial -carotene has been mainly produced using carotenogenic microorganisms, such as Blakeslea trispora [5], Rhodotorula glutinis [6], Sphingomonas sp. [7], and Phycomyces blakesleeanus [8]. One of the most promising natural commercially utilized sources of -carotene is derived from the zygomycete mold B. trispora. This heterothallic zygomycota possess two mating types, termed “plus” and “minus”, which are well known to produce -carotene on an industrial scale during the mating of (+) and (−) strain mycelia [9]. In B. trispora, -carotene biosynthesis is derived from the mevalonate biosynthetic pathway [10]. As shown in Fig. 1, the biosynthesis of -carotene from 3-hydroxy-3- methy glutarylcoenzyme A (HMG-CoA) in B. trispora requires at least five crucial enzymatic activities, which are catalyzed by enzymes encoded by the genes hmgR, ipi, carG, carRA, and carB [11–15]. While the functions of encoded genes used for -carotene biosynthesis in fungi and yeast have been identified, the regulatory mechanisms remain mostly unknown. Several studies have shown a strong correlation between the carbon source present in the medium and the amount of carotenoid biosynthesis in yeast [16]. Glucose or other fermentable sugars are metabolized through the glycolytic pathway, where a high glucose concentration serves as the carbon source and strongly inhibits carotenoid biosynthesis. Whereas, small carboxylic acids or alcohols, such as acetate or ethanol are converted to acetyl-CoA through the citric acid cycle, which is the substrate for the synthesis ofisoprenoids by the mevalonate pathway, ultimately leading to carotenoid biosynthesis [16]. However, previous studies have also reported that small quantities of acetate and short-chain carboxylic acid could stimulate zygospore production and inhibit carotene production in B.trispora, h∗
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کلمات کلیدی:
Gene expression in the regulation of carotene biosynthesis in ... link.springer.com/10.1007/s00294-007-0170-x by ERA Almeida - 2008 - Cited by 44 - Related articles The genetic and environmental factors that modify carotene biosynthesis had little or no effect on the ... A general model for the regulation of carotenogenesis in Phycomyces was derived from the results. Keywords. Transcription Blue light Sex Retinol Phthalate Mucorales .... 2000a, b), Blakeslea trispora (Quiles-Rosillo et al. Genes and Genomics - Page 262 - Google Books Result https://books.google.com/books?isbn=0080461328 Dilip K Arora, Randy Berka - 2005 - Science GENETIC REGULATION OF CAROTENOID BIOSYNTHESIS 5.1 Mucor circinelloides and ... mould for carotene fermentation is the zygomycete Blakeslea trispora (Mucorales, ... This effect is reflected in the transcriptional regulation of carG. Sparrho | Enhancing beta-carotene biosynthesis and gene transcription https://www.sparrho.com/...carotene-biosynthesis-and-gene-transcriptional-regulation... Jun 15, 2016 - The mold Blakeslea trispora is one of the most promising and ... Enhancing beta-carotene biosynthesis and gene transcriptional regulation in ... Evolution of Fungi and Fungal-Like Organisms https://books.google.com/books?isbn=3642199747 Stefanie Pöggeler, Johannes Wöstemeyer - 2011 - Science 2005) is involved in auto-regulation of carotene synthesis (Prado-Cabrero et al. ... of one of the biosynthesis genes, coding for 4-dihydromethyltrisporate dehydrogenase in ... In Blakeslea trispora as well as in Mucor mucedo and Phycomyces ... up to the level of transcriptional regulation, although occasionally with variations ... Gene expression in the regulation of carotene biosynthesis in ... www.academia.edu/.../Gene_expression_in_the_regulation_of_carotene_biosynthesis... Gene expression in the regulation of carotene biosynthesis in Phycomyces .... b), Blakeslea whose action is antagonistically regulated by genes carC trispora .... Neither stimulus modiWed the transcription little carRA mRNA, independent of ...
