دانلود رایگان مقاله لاتین اسکن صوتی میکروسکوپی از سایت الزویر
عنوان فارسی مقاله:
مطالعه مقدماتی اسکن صوتی میکروسکوپی به عنوان ابزاری برای اندازه گیری سختی شریان آئورت در بیوپسی
عنوان انگلیسی مقاله:
A pilot study of scanning acoustic microscopy as a tool for measuring arterial stiffness in aortic biopsies
سال انتشار : 2016
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بخشی از مقاله انگلیسی :
Aortic biopsy samples were obtained from 8 patients undergoing aortic bypass graft surgery for multi-vessel coronary artery disease at the cardiothoracic surgery department, Klinikum Wels-Grieskirchen, Austria. The study was approved by our regional ethics committee (Ethics committee of Upper Austria, EK number E-2-10), and all patients gave written informed consent. Details for the patients are presented in Table 1. The biopsy samples were prepared for cryosectioning by freezing in optimal cutting temperature (OCT) resin (Sakura Fintek Europe BV, Alphen aan den Rijn, The Netherlands) in pre-cooled isopentane and stored at 80 C. The OCT resin is water-soluble and does not affect the mechanical properties of the tissue. The frozen tissue blocks were subsequently sectioned to nominal 5 mm thickness sections. The sections were imaged with SAM (SAM2000 microscope, PVA TePla Analytical Systems GmbH, Herborn, Germany) as has been reported in detail previously with the Multi-Layer Phase Analysis (MLPA) method.13 This allows the determination of the acoustic wave velocity from each pixel in the image. Imaging was conducted at 760 MHz which provided a nominal spatial resolution of w1.3 mm. A series of 200 200 mm images were captured with the SAM and the mean acoustic wave speed was determined from each image.13 Each image compromised 512 512 pixels and three locations in the media were measured for each sample. Hence, the mean acoustic wave speed was averaged from 786,432 pixel measurements for each sample. For each sample, serial sections were stained with Haemotoxylin and Eosin (H&E) and imaged with optical microscopy (Nikon Eclipse 50i, Surrey, UK) to locate the medial region of the vessel for subsequent imaging with SAM (Fig. 1).
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کلمات کلیدی:
Riaz Akhtar - University of Liverpool https://www.liverpool.ac.uk/engineering/staff/riaz-akhtar/publications/ Akhtar, R., Cruickshank, J. K., Zhao, X., Derby, B., & Weber, T. (2016). A pilot study of scanning acoustic microscopy as a tool for measuring arterial stiffness in ... Search filters - OpenAIRE - Browse Publications https://www.openaire.eu/search/browse/publications?funder=wt...5... Projects: WT | Functional and evolutionary analysis of large-scale RNA ... A pilot study of scanning acoustic microscopy as a tool for measuring arterial stiffness ... Ultrasonic modeling and hydrophone measurements of dual ... - Sparrho https://www.sparrho.com/item/ultrasonic...and...measurements-of...device/489387/ Jan 9, 2015 - This paper models and experimentally measures the acoustic ... A pilot study of scanning acoustic microscopy as a tool for measuring arterial ... Brian Derby - Publications - The Academic Family Tree https://academictree.org/chemistry/publications.php?pid=76237&searchstring... 2016, Akhtar R, Cruickshank JK, Zhao X, Derby B, Weber T. A pilot study of scanning acoustic microscopy as a tool for measuring arterial stiffness in aortic ...
