دانلود رایگان مقاله لاتین آنالیز زیست شناسی از سایت الزویر
عنوان فارسی مقاله:
تجزیه و تحلیل زیست شناسی و ساختاری بخش هپارین گاوی با وزن مولکولی متوسط و بالا
عنوان انگلیسی مقاله:
Biological and structural analyses of bovine heparin fractions of intermediate and high molecular weight
سال انتشار : 2016
برای دانلود رایگان مقاله آنالیز زیست شناسی اینجا کلیک نمایید.
مقدمه انگلیسی مقاله:
1. Introduction
Heparin is a linear polysaccharide consisting mainly of →4)-- d-glucosamine-(1→4)--l-iduronic acid-(1→ repeating groups, with a complex pattern of substitution of N- and O-sulfates, and N-acetil groups (Casu, Naggi, & Torri, 2015). It acts as an anticoagulant mainly by binding to antithrombin (AT) and heparin cofactor II (HCII), enhancing the rate at which they inactivate enzymes involved in coagulation (Bourin & Lindahl, 1993; Casu, 1985). Although largely effective, the clinical use of heparin has limitations. Through its negative groups, it presents non-specific binding with many plasma proteins which affect its bioavailability. Thus, the anticoagulant effect of heparin is unpredictable, with high risk of bleeding, which requires a close clinical monitoring of the patient for a safe use (Mousa, 2007). Low molecular weight heparin (LMWH), which has a molecular weight between 3800 and 6500 Da, is obtained by enzymatic or chemical depolymerization of unfractionated heparin (UFH), which has a molecular weight between 5000 and 40,000 Da (Johnson & Mulloy, 1976; Keire et al., 2015; Linhardt, 2003). LMWH presents less non-specific binding with plasma proteins, higher bioavailability, predictable anticoagulant response and a longer plasma half-life, when compared to UFH (Cohen, 2000; Hirsh, 1998). Moreover LMWH can also be subcutaneously injected, and its use does not require a close clinical monitoring (Bratt, Törnebohm, Widlund, & Lockne, 1986; Kakkar, 2004). Heparin for pharmaceutical uses is obtained from porcine intestines or bovine lung. However, since the nineties bovine heparins are no longer used in Europe and in the United States of America because of the possibility of contamination with the prion agent of the bovine spongiform encephalopathy (BSE), related to variant Creutzfeldt–Jakob disease (vCJD) in humans (Kort, Buijsman, & Boeckel, 2005). However, there is no evidence that bovine heparin is related with BSE in humans. Bovine heparin has been continuously used in India, Brazil and Argentina, and no case of vCJD has been reported in these countries (Keire et al., 2015). Although there are many studies related to heparin, few researches have been performed with bovine heparin, probably due to the decrease of its use. Moreover, there is no commercialization of bovine low molecular weight heparin, and no studies on its possible application were found. The aim of the present investigation was to fractionate bovine heparin using a physical method (ultrafiltration), avoiding structural modifications that can be caused by chemical or enzymatic
کلمات کلیدی:
Biological and structural analyses of bovine heparin fractions of ... - خانه www.zoodyab.ir/...biological.../128190-biological-and-structural-a... - Translate this page The results showed that ultrafiltration can be used to obtain two bovine heparin fractions, which differ on their molecular weights, structural components, ... Sparrho | Biological and structural analyses of bovine heparin fracti https://www.sparrho.com/.../biological-and-structural-analyses-of-bovine-heparin.../9... Biological and structural analyses of bovine heparin fractions of intermediate and high molecular weight. Imported: 06 Oct '16 | Published: 01 Oct '16. Capillary Electrophoresis of Carbohydrates: From Monosaccharides to ... https://books.google.com/books?isbn=1607618753 Nicola Volpi - 2010 - Science ... the structural analysis of heparin and heparan sulfate in biological samples has ... [64] to analyze heparin and heparan sulfate derived from bovine kidney and ... Carbohydrates: the yet to be tasted sweet spot of immunity: https://books.google.com/books?isbn=2889196259 Deirdre R Coombe, Christopher R Parish - 2015 the most negatively charged macromolecule known in biology. ... the six fragments of heparin dp6– dp36 were prepared from bovine lung heparin, starting ... Our new structural analyses (8) proceeded in three stages: (a) First, using analytical ... Analysis of Heparins Derived From Bovine Tissues and Comparison to ... journals.sagepub.com/doi/abs/10.1177/1076029616643822 by K St Ange - 2016 - Cited by 5 - Related articles The current study examines bovine heparins prepared from both intestines and lung and compares these to porcine intestinal heparin. The structural properties ... Chemistry and Biology of Serpins - Page 346 - Google Books Result https://books.google.com/books?isbn=1461553911 Frank C. Church, Dennis D. Cunningham, David Ginsburg - 2012 - Science ... of 321 factor Xa inhibition, osmotic stress as tool in structure-function analysis, ... cells, 289 heparin binding studies, 263-264 heparin cofactor II localization in skin, ... 155–156, 263 Bovine pancreatic trypsin inhibitor, 298,330 Bovine pigment ...
