دانلود رایگان مقاله لاتین روش واحد تصفیه برای اندوگلوکاناز از سایت الزویر
عنوان فارسی مقاله:
626 روش واحد تصفیه برای اندوگلوکاناز از آسپرگیلاس نیگر توسط بارش پلی الکترولیت
عنوان انگلیسی مقاله:
Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitationسال انتشار : 2016
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مقدمه انگلیسی مقاله:
1. Introduction
Cellulases are hydrolases which comprise a complex of endoglucanase (3.2.1.4), exoglucanase and β-glucosidases (Sohail et al., 2009). Cellulases have a wide range of applications in various industries such as textile, laundry, pulp and paper, food and feed products, as well as in bioethanol production (Bhat, 2000; Dave et al., 2013; Silva et al., 2013). Cellulases have great potential in the saccharification of lignocellulosics into fermentable sugars which can be used for the production of bioethanol (Boggione et al., 2016; Maki et al., 2009; Pandey et al., 2016). It is critical to consider the conditions that contribute to enzyme stability for the design and development of a biotechnological process where the function of the enzyme is relevant. Highly polluting chemical processes can be replaced by environmentally-friendly biotechnological methodologies using cellulases. Endoglucanase is one of the main components of the enzyme complex (Sohail et al., 2009). Traditional methods for endoglucanase purification are the precipitation with ammonium sulfate, ion-exchange chromatography, affinity chromatography and gel filtration (Bakare et al., 2005; Bischoff et al., 2006; Calza et al., 1985; Gupta et al., 2012; Kaur et al., 2007; Murashima et al., 2002; Saha, 2004; Yin et al., 2010). The latter is costly, time-consuming and ammonium sulfate cannot be disposed into the environment. Besides, chromatography is not a suitable technique to be used in a downstream process from the economic point of view. Therefore, a new protocol which considers the costs and reduces the environmental impact is needed. In the downstream processing of enzymes, the choice of the purification technique should ensure the preservation of the enzyme structure associated with its biological function. One technique for large-scale protein separation involves the addition of polyelectrolytes to achieve selective protein separations. Proteins interact strongly with both synthetic and natural polyelectrolytes (Romanini et al., 2007). These interactions are modulated by a broad array of variables such as pH and ionic strength, and may result in soluble complexes (Morawetz and Hughes Jr, 1952; Xia and Dubin, 1994), complex coacervation (Ahmed et al., 1994), precipitation (Sternberg and Hershberger, 1974), or gelation (Petit et al., 1995).
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کلمات کلیدی:
Biosynthesis, purification and characterization of endoglucanase from ... www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000100008 by GT Dobrev - 2012 - Cited by 7 - Related articles Key words: endoglucanase; purification; characterization; Aspergillus niger; endoxylanase ... SDS-PAGE was performed by the method of Laemli (11) with 10.0 ... One unit of CMCase activity was defined as the amount of enzyme, forming 1 ... Single method of purification for endoglucanase from ... - INFONA https://www.infona.pl/resource/bwmeta1.element.elsevier-d384c980.../contributors Single method of purification for endoglucanase from Aspergillus niger by polyelectrolyte precipitation. María Julia Boggione, Ramiro Becher, Beatriz Farruggia. Purification of the major endoglucanase from Aspergillus fumigatus ... https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1152145/pdf/biochemj00347-0159.pdf by JB Parry - 1983 - Cited by 36 - Related articles Mar 4, 1983 - The endoglucanase (peak V) from a single ... assay methods have been described elsewhere ... One unit of enzyme is defined as the amount. [PDF]PURIFICATION OF ENDOGLUCANASE FROM Trichoderma harzianum www.pjlss.edu.pk/pdf_files/2005_1%20&%202/PJLSS_2005_40-44.pdf by M Sadia - Cited by 1 - Related articles Purification of Endoglucanase From Trichoderma harzianum ... al; 1984) according to Miller method (1959). One milliliter of enzyme solution was incubated for ... Production, Purification, and Properties of an Endoglucanase ... pubs.acs.org/doi/pdf/10.1021/jf000916p by R Lucas - 2001 - Cited by 39 - Related articles The low molecular mass (35 kDa) endoglucanase purified from cultured broths works optimally at ... by the 3,5-dinitrosalicylic (DNS) method (22), with one unit. Isolation and Characterization of a Novel Endoglucanase from a ... journals.plos.org/plosone/article?id=10.1371/journal.pone.0082437 by L Zhang - 2013 - Cited by 9 - Related articles Jump to Materials and Methods - No specific permission was required for the locations. ... Expression and purification of the recombinant cellulase Cen219. Trichoderma And Gliocladium: Enzymes, Biological Control and ... https://books.google.com/books?isbn=0203484452 Gary E. Harman, C. P. Kubicek - 2002 - Science cellulase can be substantially prevented by adding a microbial protease (e.g., ... as well as methods for purification of a single, monocomponent EGIII activity ...
