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عنوان فارسی مقاله:

ساخت و ساز ژنتیکی کلرورافیس نوترکیب سودوموناس برای بهبود به کارگیری گلیسرول

عنوان انگلیسی مقاله:

Genetic construction of recombinant Pseudomonas chlororaphis for improved glycerol utilization


سال انتشار : 2016



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مقدمه انگلیسی مقاله:

1. Introduction

Pseudomonas chlororaphis is a valuable microorganism that is capable of producing many industrially useful products. The organism is most valued as a nonpathogenic organism capable of producing the biologically active rhamnolipid biosurfactant (Solaiman et al., 2015). It can also produce the biodegradable medium-chain-length poly(hydroxyalkanoate) biopolymers (Solaiman et al., 2014). Furthermore, P. chlororaphis is a producer of several potent antifungal compounds against soil-borne plant-pathogenic fungi (Calderón et al., 2015). Commercial feasibility of P. chlororaphis to produce these microbial bioproducts, however, hinges on favorable techno-economic bioprocesses. Since the cost of fermentation feedstocks is a major contributor to the total bioproduction cost (Henkel et al., 2012; Ashby et al., 2013), many lowcost byproducts (i.e., glycerol from biodiesel production) have been studied as fermentation substrates in order to help contain production costs of microbial bioproducts (Solaiman et al., 2006). Genetic engineering is widely practiced to improve the effi- ciency of many bioprocesses that utilize various waste-streams as fermentation feedstocks. In the case of glycerol utilization in E. coli, recent advances have been documented in Mattam et al. (2013). Similar research in non-E. coli organisms, however, is much less extensive. In the Pseudomonads, for example, only the genetic system and biochemical pathway for glycerol metabolism in Pseudomonas aeruginosa was extensively studied (Schweizer et al., 1997). Even in this case, genetic engineering has not been reported to further enhance its glycerol utilization capability. In view of the potential industrial importance of P. chlororaphis, in this paper we report our study to genetically engineer a Pseudomonas species to better utilize glycerol as a sole carbon source for fermentation growth.



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کلمات کلیدی:

Genetic construction of recombinant Pseudomonas ... - USDA ARS https://www.ars.usda.gov/research/publications/publication/?seqNo115=320040 by D Solaiman - ‎2016 - ‎Cited by 1 - ‎Related articles Aug 8, 2016 - Genetic construction of recombinant Pseudomonas chlororaphis for improved glycerol utilization. Biocatalysis and Agricultural Biotechnology. Genetic construction of recombinant Pseudomonas ... - ScienceDirect 125.234.103.22/index.php?q... Genetic construction of recombinant Pseudomonas chlororaphis for ... the glycerol metabolic capability of Pseudomonas chlororaphis which is capable of ... Genetic construction of recombinant Pseudomonas chlororaphis ... - خانه www.zoodyab.ir/.../121239-genetic-construction-of-recombinant-p... - Translate this page This study is to use genetic engineering to improve the glycerol metabolic capability of Pseudomonas chlororaphis which is capable of producing commercially ... Construction of recombinant Bacillus subtilis for production of ... - NCBI https://www.ncbi.nlm.nih.gov/pubmed/16915708 Construction of recombinant Bacillus subtilis for production of polyhydroxyalkanoates. ... Pseudomonas aeruginosa/genetics; Recombinant Proteins/metabolism ... Construction of heterologous gene expression cassettes for the ... link.springer.com/article/10.1007/s00449-016-1537-5 by YH Oh - ‎2016 - ‎Cited by 1 - ‎Related articles Jan 16, 2016 - Gene-expression cassettes for the construction of recombinant ... activities of the recombinant C. beijerinckii strains expressing Pseudomonas ... Construction of recombinant pseudomonas strains harboring... - 14 ... www.bv.fapesp.br/.../construction-of-recombinant-pseudomonas-strains-harboring-diffe... Construction of recombinant pseudomonas strains harboring different PHA ... eutropha and Aeromonas hydrophila PHA synthase genes in its genome. Modern Immunosuppressives - Page 122 - Google Books Result https://books.google.com/books?isbn=3764359811 Henk-Jan Schuurman, ‎Gilles Feutren, ‎Jean-François Bach - 2001 - ‎Medical ... domain substitution with interleukin-2: genetic construction and properties of a ... cells by recombinant human CD4-Pseudomonas exotoxin hybrid protein. Cytotoxic Anticancer Drugs: Models and Concepts for Drug Discovery ... https://books.google.com/books?isbn=1461534925 Frederick A. Valeriote, ‎Thomas H. Corbett, ‎Laurence H. Baker - 2012 - ‎Medical Murphy JR, Bishai W, Borowski M et al: Genetic construction, expression and ... cells by recombinant human CD4-Pseudomonas exotoxin hybrid protein. Nature ...